Meditations Essay Example for Free

Meditations Essay Rene Descartes’ Meditations paved way to the enquiry of the epistemological nature of knowledge – a paradigm shift from an ontological priority to an epistemological priority of philosophizing. In this regard, Descartes believed that the fundamental source of knowledge is reason. As such, knowing material objects can only come upon an entity which is not external to us – that is the mind. The certainty of our understanding then rests on the clarity and distinctness of ideas constituting formal reality and not upon the immediate perception of an object. With this, he attempted to prove the existence of the Cogito, of God and dualism of the mind and the body, the methodic doubt as his primary grounding to prove the truthfulness of his claims. Let us now then analyze the seemingly problematic suppositions Descartes posits. One is dualism of the mind and body. It is because it is hard to conceive how two different things interact with each other. One indicator of such is when the mind chooses to do a specific action and the body seems to willingly do the action the mind chose. Another is when we perceive objects in the external world, our mind grasp and process the forming of these images based on our sense perception. Nevertheless, the primary question one can posit on the whole philosophy of Descartes is the appropriateness of granting existence to the conceptual entities in the mind. Existence, for Descartes, is proven through the recognition of the mind based on intuitions which manifest clarity and distinctness (or inner awareness where the mind and body are inseparable yet different from each other). By clarity, he claimed that it is the presence of an idea/object, and by distinctness, it is the relationship of clear ideas/objects and how its relationship distinguishes what belongs to an idea/object. Furthermore, he also claimed that substances are the building blocks of reality, categorized into two: the mind and the body. As such, he further maintained that these substances have primary attributes: the mind which is thought and the body, the extension. Ideas, for Descartes, are the modes of thought that link the mind and the world because they have formal and objective reality. By the former, he meant the kind of reality things have in this world and by the latter, the reality of objects represented by ideas. Hence, an idea can have formal reality because it is the mode of thought itself and it can also have an objective reality because it represents something outside of itself. In his work, one can deduce that he is certain only in his existence. As such, making him a thinking substance. He asserted that he comes to know this fact through clear and distinct perception, and logically it would follow that all his other clear and distinct perceptions are true in reference to formal and objective reality. In order to confirm the truthfulness of these clear and distinct perceptions, he proved the existence of a benign God which relies on the Mediator’s cognition of clear and distinct ideas. Even if we exempt the possibility of granting existence to fictitious entities for we may have clear and distinct perception of them, the possibility of granting existence to other conceptual entities in our mind is still questionable. First, I believe that we can only have clear and distinct perception of the conceptual entities through our senses – that is they exist in the physical realm. By that, I mean that we can have conception and understanding (on the context on how can we know them) primarily because they have manifestations in the physical world as also with regards to certain logical principles. For example, my understanding or even my idea of a tree originates from my perception of that tree in reality. Second, even if I can have clear and distinct perception of a God, it does not mean that a god/God indeed exist which cause my idea of God as what Descartes claimed. Arguably, there may be some other entity or there may be the possibility this world is just made up of matter and energy. Hence, there is no higher being which exists. It can be deduced then the appropriateness of granting existence to conceptual entities in the mind only comes if we could know their truth in the physical realm. As such, Descartes claimed of the mind being non-erroneous in granting existence to things/objects in this world is held in question.

Chromatographic Separation of Hemoglobin from Phenol Red

Chromatographic Separation of Hemoglobin from Phenol Red Syed Ibrahim Chromatographic Separation of Hemoglobin from Phenol Red by Gel Filtration Introduction From catalysis to transport, proteins take part in countless cellular processes (Nelson Cox, 2008). However, due to their small size, proteins can be challenging to work with in the lab. To overcome this hurdle, scientists have developed many fractionation techniques including centrifugation, precipitation, dialysis, and chromatography (Alberts et al., 2008). Chromatography is one of the most versatile laboratory techniques as it is used in many fields of study (Partridge, 1952; Silva et al., 2014). There are several types of chromatography, and each separates molecules based on different properties. Size-exclusion gel filtration is a type of column chromatography which resolves molecules based on their size (Duong Gabelli, 2013). In the column, there is a cross-linked polymer matrix with pores of a predetermined size (Scott, 2003). Small molecules are more likely to spend time in the pore, so they migrate slower and thus elute in later fractions (Nelson Cox, 2008). Conversely, larger molecules are less likely to enter the pore, so they migrate faster and thus elute in earlier fractions (de Jong et al., 2015). There are numerous types of column matrices, such as Sephadex G (composed of dextran and epichlorohydrin) and Sepharose (consisting of agarose) (GE, 2015). Each matrix has different sized pores and thus different ranges of molecular sizes that it can separate, called fractionation ranges (de Jong et al., 2015). In this lab, Sephadex G-25, which has a fractionation range of 1,000-5,000Da, was the column matrix (de Jong et al., 2015). Hemoglobin and phenol red were also used. Hemoglobin is a protein found primarily found in mammalian red blood cells, and is used to transport oxygen from an organism’s lungs to its tissues (Seeley, 2014). Phenol red is a chemical compound that changes color depending on the pH of the chemical environment, and so it is typically used as a pH indicator (Klein, 2012). Hemoglobin has a molecular weight of 64,500Da while phenol red has a molecular weight of 354.38Da (de Jong et al., 2015). It was expected that hemoglobin would be completely excluded from the pores of the Sephadex G-25 resin, allowing it to elute in early fractions. As well, it was expected that phenol red would be completely included in the gel, making it elute in later fractions. Purpose The goal of this lab was to separate a mixture containing hemoglobin and phenol red using size-exclusion gel filtration. As well, the aim was to compare the standard and literature wavelengths of maximum absorption of hemoglobin and phenol red. Results The results from this experiment are shown in Table 1 and Table 2. Table 1. Fraction volumes from size-exclusion gel filtration using Sephadex G25. The size-exclusion gel filtration separated a mixture of hemoglobin and phenol red. In theory, fraction A contained void volume, fraction B contained hemoglobin, fraction C contained the buffer only, and fraction D contained phenol red. Table 2. Peak absorbance and wavelength of standard hemoglobin and phenol red. Using a Beckman spectrophotometer, the wavelength profile between 380nm and 600nm of two standard samples was determined. The standard hemoglobin sample contained 250ÃŽ ¼L of 0.8% hemoglobin, while the standard phenol red sample consisted of 20ÃŽ ¼L of 0.2% phenol red. Based on the graph, the maximum absorbance values and the corresponding wavelengths were determined. Questions and Research Question 1: Search Engine: Web of Science Search Terms: hemoglobin spectroscopy Reference: DiFeo, T. J., Addison, A. W., Stephanos, J. J. (1990). Kinetic and spectroscopic studies of haemoglobin and myoglobin from Urechis caupo. Distal residue effects. Biochem. J, 269, 739-747. Search Engine: Web of Science Search Terms: hemoglobin Soret band absorptivity (sorted by relevance) Reference: Akuwudike, A. R., Chikezie, P. C., Chilaka, F. C. (2010). Absorption spectra of normal adult and sickle cell haemoglobins treated with hydrogen peroxide at two pH values. Adv. Biomed. Res, 1, 55-60. Search Engine: Google Search Terms: phenol red maximum wavelength absorption Reference: LobaChemie. (2015). Phenol Red. Laboratory Reagents and Fine Chemicals. Retrieved February 21, 2015, from http://www.lobachemie.com/pH-Indicator-05180/PHENOL-RED-CASNO-143-74-8.aspx Based on the graph from Blackboard, the wavelengths of maximum absorbance (ÃŽ »max) are shown in Table 2. For the standard hemoglobin sample ÃŽ »max = 410nm, and for the standard phenol red sample ÃŽ »max = 560nm. Based on the literature, hemoglobin and related proteins are known to give three bands of maximum absorption: ÃŽ ±-band, ÃŽ ²-band, and Soret band (DiFeo et al., 1990). For normal adult hemoglobin (HgA), the wavelength of maximum absorbance in the Soret band is ÃŽ »max = 415nm (Akuwudike et al., 2010). The wavelengths of maximum absorbance of phenol red changes based on pH. At a pH of 8.2, phenol red has ÃŽ »max = 557-560nm while at a pH of 6, phenol red has ÃŽ »max = 430-435 (LobaChemie, 2015). This change in maximum absorbance with respect to pH is understandable because phenol red is a common pH indicator, meaning it changes color based on pH (Nelson Cox, 2008). The literature and experimental wavelengths of maximum absorbance are summarized in Table 3. The experimental and literature values were fairly close, and their variance may be accounted for due to difference in the buffer and pH of the solvent. Table 3. Literature and experimental wavelengths of maximum absorbance. The experimental wavelengths of maximum absorbance (ÃŽ »max) were obtained from Table 2. The literature ÃŽ »max of hemoglobin (HgA) was based on Akuwudike et al (2010). The literature ÃŽ »max of phenol red at pH = 8.2 was based on LobaChemie (2015). Question 2: Based on the data collected in this experiment, it is impossible to determine whether the eluted hemoglobin is free of phenol red. To determine its purity, spectroscopy of the eluted hemoglobin (fraction B) would need to be conducted in order to draw a wavelength-vs-absorbance graph between 380nm and 600nm (similar to the one posted on Blackboard). If the eluted hemoglobin curve has only one peak (ÃŽ »max = 410-415nm), it contains only hemoglobin. However, it the curve has two peaks (ÃŽ »max = 410-415nm and ÃŽ »max = 557-5605nm), then the eluted fraction contains both hemoglobin and phenol red. Question 3: Search Engine: Web of Science Search Terms: size-exclusion gel filtration (sorted by relevance) Reference: Wang, Q., et al. (2005). Molecular characterisation of soybean polysaccharides: an approach by size exclusion chromatography, dynamic and static light scattering methods. Carbohydrate research, 340(17), 2637-2644. In this experiment, size-exclusion gel filtration was carried out, so hemoglobin and phenol red were separated based on size or more quantitatively based on molecular weight (Nelson Cox, 2008; Wang et al., 2005). Smaller molecules, such as phenol red, spend more time in the pores and are eluted in later fractions (Nelson Cox, 2008). Conversely, larger molecules molecules, such as hemoglobin, spend almost no time in the pores and are eluted in earlier fractions (Nelson Cox, 2008). Question 4: Search Engine: Web of Science Search Terms: chromatography theory (sorted by relevance) Reference: Lee, W. C., Tsai, G. J., Tsao, G. T. (1993). Analysis of chromatography by plate theory. Separations Technology, 3(4), 178-197. Search Engine: PubMed Search Terms: chromatography theory column length (sorted by relevance) Reference: Bedani, F., et al. (2006). A theoretical basis for parameter selection and instrument design in comprehensive size-exclusion chromatographyÃâ€" liquid chromatography. Journal of Chromatography A, 1133(1), 126-134. Based on the plate theory of chromatography, the resolution (R) is influenced by numerous factors including the plate number (N) (Lee et al., 1993). The plate number is affected by many elements of the column including column length and inner column radius (Bedani et al., 2006). Based on mathematical relationships, it has been determined that longer columns lead to increased resolution (Lee et al., 1993; Nelson Cox, 2008). Thus longer chromatography columns will separate molecules better than shorter columns. Question 5: In order to separate ÃŽ ²1,3-galactosidase (75,000Da) and ÃŽ ²1,2-xylosidase (MW 100,000Da) the Sephadex G-100 resin would be ideal. This is because the molecular weight of both enzymes is within the fractionation range of this resin (4,000-150,000Da) (de Jong et al., 2015). The heavier protein (ÃŽ ²1,2-xylosidase) will elute before the lighter protein (ÃŽ ²1,3-galactosidase). Question 6: The fractionation range of Sephadex G-100 is 4,000-150,000Da (de Jong et al., 2015). Alcohol dehydrogenase (MW 140,000Da) falls within this range, while aldehyde dehydrogenase (MW 200,000Da) is above the range. Since both enzymes are not on the same extreme of the fractionation range, aldehyde dehydrogenase will elute very quickly and alcohol dehydrogenase will elute shortly after. Thus, Sephadex G-100 can be used to resolve alcohol dehydrogenase and aldehyde dehydrogenase. However, it would be ideal to use Sephadex G-200 because the molecular weight of both enzymes would be within the fractionation range of 5,000-600,000Da (de Jong et al., 2015). Question 7: Search Engine: PubMed Search Terms: â€Å"fast protein liquid chromatography† (sorted by relevance) Reference: Sheehan, D., O’Sullivan, S. (2004). Fast protein liquid chromatography. In Protein purification protocols (pp. 253-258). Humana Press. Search Engine: PubMed Search Terms: â€Å"fast protein liquid chromatography† (sorted by relevance) Reference: Goke, B., Keim, V. (1992). HPLC and FPLC. Recent progress in the use of automated chromatography systems for resolution of pancreatic secretory proteins. International Journal of Pancreatology, 11(2), 109-116. Search Engine: PubMed Search Terms: â€Å"fast protein liquid chromatography† (sorted by relevance) Reference: Lavanya, G., et al. (2011). Protein Chromatography. J Biomed Sci and Res, 3(3), 424-438. Search Engine: Google Search Terms: fast protein liquid chromatography Reference: Bio-Rad. (2015). Fast Protein Liquid Chromatography. Bio-Rad Life Science Research.Retrieved February 22, 2015, from http://www.bio-rad.com/en-ca/applications-technologies/fast-protein-liquid-chromatography Search Engine: PubMed Search Terms: fast protein liquid chromatography use Reference: Verbeke, K., Verbruggen, A. (1996). Usefulness of fast protein liquid chromatography as an alternative to high performance liquid chromatography of 99m Tc-labelled human serum albumin preparations. Journal of pharmaceutical and biomedical analysis, 14(8), 1209-1213. Fast protein liquid chromatography (FPLC) is an automated chromatography technique that uses columns with a small inner diameter (Goke Keim, 1992; Sheehan O’Sullivan, 2004). By means of pumps and valves, the FPLC system regulates how fast the liquid mobile phase moves down the agarose stationary phase a constant, and maintains a constant flow rate (Lavanya et al., 2011). As well, based on the readings of the UV detector and conductivity meter, the FPLC system is able to control how and when the eluted fractions should be separated (Bio-Rad, 2015). There are variations of FPLC that allow proteins to separated base on size, charge or hydrophobicity (Verbeke Verbruggen, 1996). FPLC, as its name suggests is quite useful in analyzing and purifying protein mixtures (Lavanya et al., 2011). For example, FPLC has been used to identify zinc-containing proteins in rat saliva; and it has also been used to separate and analyze pancreatic juice in order to examine disease-causing proteins (Lavanya et al., 2011). Conclusion In this lab, a mixture of hemoglobin and phenol red was resolved using sizeexclusion gel filtration. Since Sephadex G-25 (fractionation range 1,000-4,000Da) was used as the column matrix, it was expected that hemoglobin would be completely excluded from the matrix pores, allowing it to elute in early fractions. As well, it was anticipated that phenol red would be completely included in the gel, making it elute in later fractions. The hemoglobin/phenol red mixture was separated into four fractions and their respected volumes are shown in Table 1. In theory, fraction A contained void volume, fraction B contained hemoglobin, fraction C contained the buffer only, and fraction D contained phenol red. Due to time and budget constraints, the absorbance of the eluted fractions was not analyzed. However based on an absorbance-vs-wavelength graph, the wavelengths of maximum absorbance (ÃŽ »max) for standard hemoglobin (ÃŽ »max = 410nm) and standard phenol red (ÃŽ »max = 560nm) were determined, as described in Table 2. These values were also compared to literature values, as shown in Table 3. Normal adult hemoglobin (HgA) is known to have maximum absorbance at ÃŽ »max = 415nm, while phenol red is known to have ÃŽ »max = 557-560nm. The experimental and literature values were fairly close to one another, and their slight variance may be accounted for due to difference in the buffer used and pH of the solvent. For further research, spectroscopy of the eluted hemoglobin (fraction B) would need to be conducted in order to draw a wavelength-vs-absorbance graph between 380nm and 600nm. This would help to clarify whether the eluted hemoglobin is free of phenol red. References Akuwudike, A. R., Chikezie, P. C., Chilaka, F. C. (2010). Absorption spectra of normal adult and sickle cell haemoglobins treated with hydrogen peroxide at two pH values. Adv. Biomed. Res, 1, 55-60. Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2008).Molecular Biology of the Cell(5th ed.). New York: Garland Science. Bedani, F., et al. (2006). A theoretical basis for parameter selection and instrument design in comprehensive size-exclusion chromatographyÃâ€" liquid chromatography. Journal of Chromatography A, 1133(1), 126-134. Bio-Rad. (2015). Fast Protein Liquid Chromatography. Bio-Rad Life Science Research. Retrieved February 22, 2015, from http://www.bio-rad.com/en-ca/applications-technologies/fast-protein-liquid-chromatography de Jong, M., Botelho, R., Antonescu, C., Wakarchuk, W. (2015).Laboratory Manual: Biochemistry- BCH261(Winter 2015 ed.). Toronto: Ryerson University. DiFeo, T. J., Addison, A. W., Stephanos, J. J. (1990). Kinetic and spectroscopic studies of haemoglobin and myoglobin from Urechis caupo. Distal residue effects. Biochem. J, 269, 739-747. Duong-Ly, K. C., Gabelli, S. B. (2013). Gel filtration chromatography (size exclusion chromatography) of proteins. Methods in enzymology, 541, 105-114. GE. (2015). Product Catalog. GE Healthcare Life Sciences. Retrieved February 21, 2015, from http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/ GELifeSciences/products/products_by_technology/ Goke, B., Keim, V. (1992). HPLC and FPLC. Recent progress in the use of automated chromatography systems for resolution of pancreatic secretory proteins. International Journal of Pancreatology, 11(2), 109-116. Klein, D. R. (2012). Organic chemistry (2nd ed.). Hoboken, N.J.: John Wiley. Lavanya, G., et al. (2011). Protein Chromatography. J Biomed Sci and Res, 3(3), 424-438. Lee, W. C., Tsai, G. J., Tsao, G. T. (1993). Analysis of chromatography by plate theory. Separations Technology, 3(4), 178-197. LobaChemie. (2015). Phenol Red. Laboratory Reagents and Fine Chemicals. Retrieved February 21, 2015, from http://www.lobachemie.com/pH-Indicator-05180/PHENOL-RED-CASNO-143-74-8.aspx Nelson, D., Cox, M. (2008). Lehningers Principles of Biochemistry (5th ed.). New York, N.Y.: WH Freeman and Company. Partridge, M. W. (1952). Chromatography and its applications in pharmacy. Journal of Pharmacy and Pharmacology, 4(1), 217-230. Scott, R. P. (2003). Principles and practice of chromatography. Chrom-Ed Book Series, 1. Seeley, R. (2014). Seeleys Anatomy Physiology (10th ed.). New York, NY: McGraw-Hill. Sheehan, D., O’Sullivan, S. (2004). Fast protein liquid chromatography. In Protein purification protocols (pp. 253-258). Humana Press. Silva, J., Teodoro, J., Afonso, R., Aquino, S., Augusti, R. (2014). Photolysis and photocatalysis of ibuprofen in aqueous medium: characterization of byà ¢Ã¢â€š ¬Ã‚ products via liquid chromatography coupled to highà ¢Ã¢â€š ¬Ã‚ resolution mass spectrometry and assessment of their toxicities against Artemia Salina. Journal of Mass Spectrometry, 49(2), 145-153. Verbeke, K., Verbruggen, A. (1996). Usefulness of fast protein liquid chromatography as an alternative to high performance liquid chromatography of 99m Tc-labelled human serum albumin preparations. Journal of pharmaceutical and biomedical analysis, 14(8), 1209-1213. Wang, Q., et al. (2005). Molecular characterisation of soybean polysaccharides: an approach by size exclusion chromatography, dynamic and static light scattering methods. Carbohydrate research, 340(17), 2637-2644.

All and all another brick in the wall Essay -- Music, Pink Floyd

Pink Floyd’s album The Wall influenced a great change in the music industries from the 1980s to today within America. The album released on Nov. 30, 1979, the live concert preformed in 1980 through 1981, and the movie released in 1982 gave the foundation to the 1980’s American music scene to the present music scene. The album The Wall brought back a vibe of psychedelics from the 1960s but gave a twist of hard rock from transitioning electronics to guitars. The concert for The Wall was the first live show to adopt media effects, such as lighting, and props as a main part of a live performance which changed all rock stars’ performances forever. The concert helped to create the most popular genre of the 1980s, glam metal. Pink Floyd literally pushed the edge of what concerts were before with their spectacular visuals. Pink Floyd’s The Wall was a pioneer in producing promotional films for albums or songs with the movie. Making a movie for a album was a n ew idea of using media to sell one’s music and influenced a great change in what was important visual or lyrics. The album The Wall by Pink Floyd literally helped to create the 1980’s of America which in turn leads to creating today’s music industry of America. Generally in the Untied States a average teenager growing up in the mid 70’s through the 80’s was shown a span of different genres and cultural changes. Many teenagers were becoming in need of something new for the 1970s was a time of many experimental genres. When interviewing a man who grew up in the 1970s and 1980s, Pink Floyd was a very recognized band. When asked what his thoughts were on the album The Wall he explained how it was a new version of music that amazed him and all his friends. He states â€Å"This so ... ...iety. Pink Floyd movie help to inspire artist to explore different media to express songs. November 30, 1979 would surely be the greatest day or one of the most important in America for it changed and gave the idea to merge media with music. From this merge the idea of product placement and illegal downloading have changed the music business. The profit is now not found in recording but in concerts for musicians. Pink Floyd’s concert help create the typical idea of what a concert needs to keep the audience entertained. The album The Wall by Pink Floyd created the music culture of present day America. This album can be explained it one lyric â€Å"All and All just another Brick in the Wall† from the song â€Å"The Wall† by Pink Floyd. This literally explains this band, album, concert and movie was just another building block in the creation of Americas’ music industry. All and all another brick in the wall Essay -- Music, Pink Floyd Pink Floyd’s album The Wall influenced a great change in the music industries from the 1980s to today within America. The album released on Nov. 30, 1979, the live concert preformed in 1980 through 1981, and the movie released in 1982 gave the foundation to the 1980’s American music scene to the present music scene. The album The Wall brought back a vibe of psychedelics from the 1960s but gave a twist of hard rock from transitioning electronics to guitars. The concert for The Wall was the first live show to adopt media effects, such as lighting, and props as a main part of a live performance which changed all rock stars’ performances forever. The concert helped to create the most popular genre of the 1980s, glam metal. Pink Floyd literally pushed the edge of what concerts were before with their spectacular visuals. Pink Floyd’s The Wall was a pioneer in producing promotional films for albums or songs with the movie. Making a movie for a album was a n ew idea of using media to sell one’s music and influenced a great change in what was important visual or lyrics. The album The Wall by Pink Floyd literally helped to create the 1980’s of America which in turn leads to creating today’s music industry of America. Generally in the Untied States a average teenager growing up in the mid 70’s through the 80’s was shown a span of different genres and cultural changes. Many teenagers were becoming in need of something new for the 1970s was a time of many experimental genres. When interviewing a man who grew up in the 1970s and 1980s, Pink Floyd was a very recognized band. When asked what his thoughts were on the album The Wall he explained how it was a new version of music that amazed him and all his friends. He states â€Å"This so ... ...iety. Pink Floyd movie help to inspire artist to explore different media to express songs. November 30, 1979 would surely be the greatest day or one of the most important in America for it changed and gave the idea to merge media with music. From this merge the idea of product placement and illegal downloading have changed the music business. The profit is now not found in recording but in concerts for musicians. Pink Floyd’s concert help create the typical idea of what a concert needs to keep the audience entertained. The album The Wall by Pink Floyd created the music culture of present day America. This album can be explained it one lyric â€Å"All and All just another Brick in the Wall† from the song â€Å"The Wall† by Pink Floyd. This literally explains this band, album, concert and movie was just another building block in the creation of Americas’ music industry.

Film Analysis of All That Heaven Allows Essays -- Papers Movie Film Ci

Film Analysis of All That Heaven Allows Chosen sequence: Golden Rain Tree/Cary's bedroom scene. Before the emergence of 'auteur theory' the director Douglas Sirk was a renowned exponent of classical Hollywood narrative, particularly in the genre of romantic melodrama, of which his film All That Heaven Allows is a classic example. However, he is now regarded as a master of mise-en-scene, one of the few tools left to a director working within the constraints of the Hollywood studio/institutional system who is now thought to have been highly critical of American mainstream culture and society in this prosperous era. 1, 2 The 'Golden Rain Tree' sequence occurs early on in the film after the opening panoramic, establishing shot - showing the scene of the action, a small middle-class New England town in autumn. The main protagonists are soon introduced of which the prime causal agent is an unsettled woman, Cary Scott (Jane Wyman), in keeping with romantic melodrama. As a widow, she is a victim of circumstance who is eager to change her life. Her friend visits (Mona) and hopes to persuade Cary to take a conventional route out of widowhood but, by chance, she meets the gardener, Ron Kirby (Rock Hudson) and from the mise-en-scene in these opening scenes it is obvious that she would prefer a romantic affiliation of some sort with Ron. After a few pleasantries, some tea at the table outside Cary's house and some profound references to gardening, Ron goes to hand Cary a small brance/twig, evidently a token of deep affection. The film so far displays all the conventions of classical narrative and maintains all the dominant ideologies o... ...lassical narrative cinema. In Being There, the character and motives of Gardiner are made much clearer to the viewer through the imaginative use of mise-en-scene, as illustrated above. NOTES 1. Carroll. Essay The Moral Ecology of Melodrama: The Family Plot and Magnificent Obsession. p. 170. 2. Cook. p. 76-79. BIBLIOGRAPHY An Introduction to Film Studies Jill Nelmes (ed.) Routledge 1996 Anatomy of Film Bernard H. Dick St. Martins Press 1998 Key Concepts in Cinema Studies Susan Hayward Routledge 1996 Teach Yourself Film Studies Warren Buckland Hodder & Stoughton 1998 Interpreting the Moving Image Noel Carroll Cambridge University Press 1998 The Cinema Book Pam Cook (ed.) BFI 1985 FILMOGRAPHY All That Heaven Allows Dir. Douglas Sirk Universal 1955 Being There Dir. Hal Ashby 1979

Little Fugue and Morning Song by Sylvia Plath Essay -- Sylvia Plath Po

A relationship is an emotional connection to someone involving an interaction between two or more people. There are many types of relationships, some functional and others far from being workable. I will demonstrate this through my texts of; Little Fugue, and Morning Song both poems written by Sylvia Plath; the movie, Love Actually; and the book, Trickster’s Choice by Tamora Pierce. Little Fugue by Sylvia Plath is my first example of how we all perceive our different relationships. This poem is about Plath talking of her father and herself and the lack of communication between the two. Throughout the poem, Plath contradicts herself, saying, ‘I was seven, I knew nothing’ yet she constantly talks of the past, remembering. Her tone is very dark and imposing, she uses many images of blindness, deafness and a severe lack of communication, ‘So the deaf and dumb/signal the blind, and are ignored’. Her use of enjambment shows her feelings and pain in some places, in other places it covers up her emotional state. She talks of her father being a German, a Nazi. Whilst her father may have originated from Germany, he was in no way a Nazi, or a fascist. He was a simple man who made sausages. ‘Lopping the sausages!’ However she used this against her father, who died when she was but eight, saying that she still had night mares, ‘They color1 my sleep,’ she also brings her father’s supposed Nazism up again, ‘Red, mottled, like cut necks./There was a silence!’. Plath also talks of her father being somewhat of a general in the militia, ‘A yew hedge of orders,’ also with this image she brings back her supposed vulnerability as a child, talking as if her father was going to send her away, ‘I am guilty of nothing.’ For all her claims of being vul... ...r child being an alien, she still stumbles from bed ‘cow heavy’ at a single cry from the child. Morning Song is literally the cry of a baby, as it calls for it’s mother. The relationship between mother and child is strong although Plath seems to view her child as something totally unchildlike. She doesn’t seem to be able to connect with her child in any way. I have learned that relationships are diverse and can change from one moment to the next. I have learned that not all people share the same views as I do when it comes to the people I hold dear. The world around me is a very different place to my perceived ideals. Relationships are like diamonds, with many sides and facets. It can be perfect and clear, or cloudy and distorted. It is life. 1American spelling used as it is a direct quote 2Corus is the capital city of Aly’s home country, Tortall

“Hip-Hop, The Renaissance” Essay

They say â€Å"they never really miss you til you dead or you gone† So on that note I’m leaving after the song. A wise MC by the name of Shawn Corey Carter told me this as his Black Album gave me identical eclectic vibes I felt listening to the likes of Digable Planets, Big L, Big Daddy Kane, Big Pun, Common, Mos Def and others artists born of â€Å"The Renaissance†. I have come to inform you all of this renaissance known as hip-hop, a renaissance that will never cease breathing until the last listener’s foot is finished tapping to the rhythm and rhyme scheme of their favorite record. Hip-Hop, a genre of stylized rhythmic music that is used to accompany a rhythmic form of speech that would go on to become rap. Many have tried unsuccessfully to pinpoint the location of the birth of this â€Å"Renaissance† by saying that this is a West-coast, Midwest, and even a down South born style of music. However, I prefer to take you back to the 1970’s on 1520 Sedgwick Street in the Bronx, the home of Jamaican DJ Kool Herc who is arguably considered the father of hip hop, if not one of the many. See Kool Herc’s style of deejaying at house parties was that he would recite rhymes over what would in later time garner the title â€Å"an instrumental†. He would use a plethora of in house reference when dropping said rhymes on the microphone not knowing this would spawn a movement that would travel through the streets of Brooklyn and be spread by various other party Dj’s as well such as Coke La Rock. Spoken- word and music by early founding fathers such as Gil Scott- Heron, Jalal Mansur, and The Last Poets (one of whom I know personally) helped this movement come into fruition because of the simple fact that their lyrics were based on post- civil rights era life in the urban community. Oh yes the movement was real. Not only because everyone could relate but because it came via a rhythmic delivery that captured the mind and imagination of the people of that time. In creating this movement, they led us into a new era of Hip-Hop in which The Sugarhill Gang recorded the first record of the genre in 1979 titled â€Å"Rapper’s Delight†. There were many others who allegedly stated their claim as being the first, but couldn’t contend with Sugarhill Gang because â€Å"Rapper’s Delight† made the Billboard Top 100. It’s like Rosa Parks. We all know she wasn’t the first to not give up her seat, but why argue if it led to a positive result in the grand scheme of things. By the 80’s, Hip-Hop had spread to every major city in the United States, but Philadelphia was the only city that could come close to drawing comparison to New York’s contributions to Hip-Hop after Philly artist Jocko Henderson released his record â€Å"Rhythm Talk† Since I have told you the history of the Renaissance known as Hip-Hop, let’s bring it to the present since every student in here, I’m assuming, is a late 80’s, 90’s baby.   Now let’s talk about present day artists that epitomize the rhythmic, eclectic and dynamic styles. We talk about Jay-Z, Kanye West, Drake, Meek Mill, Big Sean, Eminem, Common, Mos Def, and so on. I prefer to talk about Black Thought’s team, The Roots. This Hip-Hop group has transcended the Hip-Hop genre since the came on to the scene in the City of Brotherly Love in 1987. Black Thought and ?uestlove both attended the Philadelphia High School for Creative and Performing Arts where ?uestlove would be the drum kit at talent shows while Black Thought laced the percussions with lyrics that would make The Last Poets, Gil Scott-Heron, and especially DJ Kool Herc proud of the fact that the roots of Hip-Hop have not been forgotten. Ironic how they would become known as The Roots in the process of their formation. They barely missed the Top 100 when they dropped their first major label album titled â€Å"Do You Want More?!!!!† in January 1995. What made this so significant is that they used no prerecorded instruments, kicks, or sounds. Every bit of their sound was original. This to me is why they epitomize what Hip-Hop because they are as original in the 21st century as they were in the 90’s when they made their debut. Another artist I will mention is 23 year old Compton emcee Kendrick Lamar who just released his debut album â€Å"good kid, m.A.A.d city†. I believe it is the album of the year, but that’s a persuasive speech for another day. With this one album alone Kendrick shows veterans in the game how to perfectly arrange an album and turn it into a masterpiece that reaches every generation and demographic. In closing I will say that what started as a Renaissance has become commercialized. Do I feel that there is hope that we will one day as artists go back to our roots like The Roots and Kendrick Lamar? Yes, but it will be a strenuous process. The Renaissance is in good hands with artists like Kendrick Lamar making classics with one debut. Not to mention myself. I try to bring a new feel myself to the game as well. Hip-Hop, The Renaissance is taking a step into the next generation. I will leave you Hip-Hop fans with this question: â€Å"Will you take that next step with me.

Hunting life Essay

Throughout the pastoral poem, there are several different symbols. The symbols I used were deer, dreams and fear. The deer symbolizes a trophy. I used it as a trophy because if you get your winning deer then that means you have gotten out of this small town and moved onto bigger and better things. Another symbol was dreams because the only way to be able to follow your dreams is to actually be successful and get away from the town/area that is holding you back. Last, I used fear because you get scared to actually take control of your future and to take control  of your dreams to get away and be successful in life without being judged or held back. These symbols were used throughout the entire pastoral poem and relate to real life. Death and Mourning The theme that was used in my pastoral poem was death and mourning. Death is used by the symbolization of the deer. Even though the deer did not physically die, it shows the death of getting out of town. Being in this small town shows that you cannot go out and see what you want to see or pursue your dreams because you are stuck. This is causing you to die on the inside. Mourning is incorporated into this pastoral poem by dreading not â€Å"killing† the deer. This is because if you would have just killed it you would be out of this town but now you really are stuck until you â€Å"kill† it. Death and mourning is shown throughout this pastoral poem as the theme. Grove 2 Hunting Life Silence all around deep in the woods. Isolated for the good sitting in my tree stand, Up in the tree stand waiting for my deer,But after several days I am beginning to fear. Aside from the fear, the fear of not getting my deer, I think about life and embrace silence, While dreaming about succeeding as I breathe in the cool air. As I sit in my tree stand I finally think I hear a deer. Slowly I raise the gun to my chin, getting him centered in my sight. That kill shot is right there. I cannot help but to grin because I have been waiting so long. Waiting so long in my tree stand for my deer. So I take a deep breath, let it out and squeeze. Adrenaline has now gotten the best of me. Shaking, thinking and hoping to please, And then I see the monster is instantly down. I fly down my stand, run to his side and kneel in the blood, He is my 10 point prize. But then I realize this is just a dream, The dream of actually getting anywhere. Grove 3 In this town you can only dream, Dream of succeeding and getting away from this life. Silence is always the key, Keep quiet, breathe in the cool air and maybe you will get your prized deer.